PUNCTA

The recognition and analysis of distinct point structures, within a given field of view.

Puncta in the nucleus and in the cytoplasm

green = original NeuN image
blue = original SOX2 image (enhanced signal) contours:
grey = NeuN cells
red = “SOX2 positive” NeuN cells

gray = puncta on green channel
blue = puncta on green channel having already signal in red channel